Can uvc rays penetrate a petri dish








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Uv Radiation Damage And Repair Lab Flashcards

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The effects of ultraviolet light on escherichia coli

However the further action towards the use of UV-C radiation to sanitize fish meals seems to be justified. Only limited information about the effect of UV-C on bacterial spores in food products and animal feeds is available in literature. The probable number of all of the studied bacteria was determined with the MPN method in a 3-tube set, making tenfold dilutions of the radiated samples in appropriate media. The observed differences connected with the meal type were not statistically significant , with the exception of bacteria of the genus Enterococcus Table 6. H7 inoculated onto RTE ready-to-eat foods product was 0. By contrast, the lowest UV-C dose Changes in the number of Clostridium sporogenes spores depending on the surface density of the UV-C radiation dose. The highest inactivation rate, however, was observed in the case of enterococci. After the incubation, characteristic black colonies of Clostridium sporogenes were counted on each plate. Among other factors, which may influence the efficiency of spore inactivation, the authors mentioned their initial concentration and identified the bacteria species level. Another possible cause may be some differences in the initial concentration of bacteria and spores in both types of fish meal.

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3-UVC has wavelengths of nm and is the most penetrating and dangerous Now that we have discussed the categories of UV rays, we will give the rest of our In the lab experiment today, we want to find the effects of UV irradiation on two species colonies, if grown at 30°C or lower temperatures on agar plates.

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Description: The efficiency of the studied methods was evaluated based on the elimination rate of Salmonella , E. Final identification was performed using the serological Phadabac Strep Test. Among the benefits resulting from the application of UV-C for decontamination, the most essential are the simplicity of the method, low dependency on pH and temperature, the lack of any toxic chemical substances applied, and the lack of byproducts and remains generated in the case of chemical reactions [ 7 , 8 ]. Some differences between bacterial species were observed with respect to their resistance to UV-C radiation. Changes in the number of Clostridium sporogenes spores depending on the surface density of the UV-C radiation dose. The bactericidal effect of UV radiation is most of all due to the photochemical destruction of nucleic acids, resulting in the inhibition of cell reproduction. However, further detection of bacterial contaminations resulted in the conclusion that a secondary contamination of this material had to be considered, which occurred probably during transport or processing. In this study spores of Clostridium sporogenes showed a considerably higher resistance to UV-C radiation than Salmonella , E. The initial investigations did not prove the presence of the chosen indicator bacteria or that of further fungi or Actinobacteria in the samples. The developmental stage of microorganisms subjected to inactivation is also of essential importance for the effectiveness of most sanitizing technologies. Only limited information about the effect of UV-C on bacterial spores in food products and animal feeds is available in literature. The values of surface fluencies of a dose were obtained by selecting appropriate exposition times, which were calculated according to the equation where is the time of exposition sec. A similar susceptibility was observed in many studies concerning the effect of UV-C radiation on microorganisms of faecal origin [ 8 , 16 ]. Investigations carried out on the molecular level proved that the degree of spore dehydration and the structure of peptidoglycan in the cortex, which largely determine the thermoresistance, play a relatively small role in the induction of spore resistance to UV irradiation.
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